Principal Investigator:

Robert J. Lascano
Texas A&M University
USDA-ARS
3810 4th Street
Lubbock, TX 79415
E-mail: r-lascano@tamu.edu

Cooperators:

Lubbock: Hong Li, Jill Booker, Kevin Bronson, Wayne Keeling

Beaumont: Ted Wilson

Primary Research Location: Lamesa and Lubbock, TX

Project Title: Cotton, Water, Nitrogen and IPM in Precision Agriculture


Reporting Period: 1 September 1997 - 31 August 2000

Objectives:

1. To determine the water and nitrogen balance within large agricultural fields. This involves both experimental and a modeling of the nitrogen cycle as it relates to water and nitrogen management in relation to cotton lint yield.

2. To determine the spatial and temporal variability of N within a large field.

3. To assess the use of remote sensing for site-specific management of cotton, and detection of multiple stresses in cotton.

4. Integrate the effect of N, H20, and insect injury on growth, development, maturation, and yield of cotton.

5. Develop decision support software that predicts the site-specific impact of multiple stresses on cotton crop growth, development, maturation, and yield.

A. Summary of Progress

Objective 1 and 2: Quantify the spatial and temporal variability of factors that can be addressed by precision agriculture practices.

Topographic land features may cause spatial variability in soil water, particle-size, and N and P distribution resulting in variable crop yields across the field. Topographic features such as surface elevation influence the spatial distribution pattern of organic C, particle-size, soil water, inorganic N, and crop yield. Surface elevation and curvature, soil organic matter, P, and K contents strongly contributed to spatial and temporal variability of maize yield on a hill slope in NY State, and it has been suggested to use a topographic parameter such as surface elevation and curvature to determine spatial patterns of yield and soil erosion. Further, soil physical and chemical properties should be measured as a function of space and time, and spatial-domain methods based on state-space models are appropriate to describe spatial relationship between soil water, nutrient and crop variables. Understanding the relationship between soil properties, topography, and irrigation and fertilization practices would be a basis for increasing cotton lint yield on the Texas High Plains. There is a need of providing a complete deterministic explanation of the interdependence between irrigation and fertilization practices and local field conditions. It was hypothesized that plant water and N use and cotton lint yield were related to irrigation and N supply and topography. Objectives of the study were to (i) determine the spatial pattern of soil water and N distribution, N uptake by cotton, and lint yield, (ii) quantify the effects of irrigation and N fertilization on response variables, (iii) quantify lint yield, soil property, and topography underlying processes using state-space approach, and (iv) compare the state-space forecasting lint yield data with the following year measured lint yield.

MATERIALS AND METHODS

Site characteristics and experimental setup
The study started in May 1998 at the Lamesa Agricultural Research Farm of Texas A&M University. Altitude at the site declines from W to E and downward from S toward the center, then gently rolling upward to the N. The soil is structureless, well drained, highly erodible, and classified as an Amarillo sandy loam. In general, the soil has a low electrical conductivity, a moderate permeability, a moderate water-holding capacity, and a highly calcareous substratum. The experimental area was 32 m wide and 700 m long. Treatments consisted of irrigation at rates of 50% and 75% calculated cotton evapotranspiration (ET), and N fertilization at rates of 0, 90 and 135 kg ha-1, respectively. There were 5 replications of N treatments arranged in an incomplete block design. Neutron access tubes were installed 25 m apart for each irrigation level. Plot size was 16 1-m rows wide and 50 m long. Cotton (Gossypium hirsutum L., 'Roundup® Ready 2326') was seeded at a rate of 16.8 kg ha-1 on 8 May in 1998 and on 10 May 1999. To ensure plant emergence, the center pivot applied 13 mm of water immediately after seeding. Nitrogen fertilizer (urea, 32-0-0) was fractionally applied by a chisel into the soil at a rate of 45 kg ha-1 at emergence, bloom, and first-square. Herbicides, insecticides and fungicides were applied according to regional recommendations.

Irrigation and soil water monitoring
Irrigation treatments were applied by LEPA. Water was applied according to rain at different growth stages every 3 d. Total water applied was 242 and 323 mm in 1998, and 190 and 286 mm in 1999 for the 50% ET and 75% ET irrigation, respectively. Soil water was monitored throughout the growing season by neutron attenuation.

Plant and soil sampling and analysis
Latitude, longitude, and altitude at each measurement site were taken using a DGPS receiver. Cotton rooting depth was measured in August at three locations per irrigation level. Soils were sampled at emergence, bloom, and harvest to determine soil NO3-N and P variability. Samples were taken within a 0.5-m circle from each neutron access tube to a depth of 1.8 m. Total N uptake by cotton was measured on a 10 d interval starting two weeks after emergence until harvest. Ten plants per plot were taken at vegetative stage and 4 plants per plot were taken during bloom and boll stages. At each plot plant density was measured three times at vegetative stage.
Soil samples were air-dried and sieved to 2 mm. Soil texture was measured using the hydrometer method. The 0.1M KCl extractable NO3-N and acidified ammonium acetate-EDTA extractable P was measured using a Technicon Auto-Analyzer. Root length, fresh plant biomass, and dry matter of roots, leaves, stems, bolls, and seeds were measured with samples dried at 70°C for 48 h, and ground to a 40-mesh size. Plant N concentrations were determined using a N analyzer. Cotton plants were desiccated with Roundup® 10 d before harvest. Cotton lint was hand harvested on 5 October in 1998 and on 12 October in 1999. Each plot lint yield was determined from four 1 x 4 m areas 3 m apart.

Mixed model, crosscorrelation and state-space analysis
Mixed model analysis was used to determine effects of irrigation and N fertilization on response variables. Regression relationships and linear correlation among the soil and cotton crop variables was determined. The spatial correlation between two variables was determined with the crosscorrelation covariance and the crosscovariance function. Cotton lint yield, soil water, texture, and site elevation were identified in state-space analyses as quadrivariate first-order autoregressive processes. We used a state-space procedure to determine the mean matrix parameters.

RESULTS

Spatial and temporal pattern of soil water distribution
Soil water distribution at different soil layers varied with irrigation level, soil depth, site elevation, and slope length across the field, as shown in Fig. 1. Water content changed consistently across the field and water distribution pattern at all soil layers showed a dependence on site elevation and slope length (Fig. 1). Along the 50% ET (Fig. 1ac), the soil water content in the rooting zone was higher (0.148 m3 m-3 on average) on the south-center lower position area. Water contents changed to lower (0.123 m3 m-3 on average) on the northern extended upslope (Fig. 1ac). Along the 75% ET plots (Fig. 1bd), movement of water showed a similar trend and changes were consistent across the lower area and upslope. Surface soil water content was especially low between 500-550 m on the 75% ET plots (Fig. 1bd), where the footslope area had a higher sand content compared to the sand content on the shoulder area.


Fig. 1. Spatial pattern of soil water content at different depths in the rooting zone (0-0.9 m) related to site elevation. Data were measured on 24 June 1998.

In 1998, the temporal soil water distribution pattern decreased from June to August then increased again towards harvest in October. Analysis of variance indicated that there was a significant difference in the temporal assessments of the soil water contents between measurement time (date), irrigation level, and soil depth (results not shown).

Spatial pattern of plant water use, N uptake, and lint yield

Similar to the soil water distribution, soil NO3-N in the rooting zone (0-0.9 m) was high on lower positions in middle of the field (Fig. 2ab). Unlike the soil water distribution, clay content was high on the northern upslope and shoulder area (Fig. 2ab). Total N uptake by cotton was 178 and 220 kg ha-1 in 1998, and 182 and 209 kg ha-1 in 1999 at the 50% and 75% ET, respectively, which showed a close dependence on irrigation (Fig. 2cd). Cotton lint yield varied with irrigation level and changed within a short distance along transects (Fig. 2ef). Mean cotton lint yields were 704 and 962 kg ha-1 in 1998, and 801) and 924 kg ha-1 in 1999, respectively at 50% and 75% ET irrigation level. Correspondingly, high yields were linked to the low positions in center field areas (Fig. 2ef), where soils contained higher water content within the rooting zone (Fig. 1). Lint yields in 1999 declined linearly from the middle of the field to northern upslope areas, especially on the 50% ET where the slope length was extended (Fig. 2e).


Fig. 2. Spatial pattern of soil clay (0-0.3 m) and NO3-N (0-0.9 m) measured in July 1998, and cotton lint yield and N uptake measures at harvest 1998 and 1999.

Cross-correlation between Lint yield, soil water, texture, and topography
There was a cyclic, positive or negative, feedback relationship between lint yield, soil water, and elevation measured in 1998. The crosscorrelation functions of the cotton lint yield, volumetric soil water, clay, sand, and site elevation ranged between -0.6 and 0.6. As illustrated in the scatter diagram (Fig. 3), the lint yield was positively cross-correlated with soil water, but negatively cross-correlated with elevation (Fig. 3c) on the 50% ET transect at a lag distance of ± 30 m. Lint yield was negatively cross-correlated with clay (Fig. 3b) but positively with sand content (Fig. 3f) across a distance of 80 m. The crosscorrelation distance between clay and elevation was ± 40 m on the 50% ET transect (Fig. 3d), where clay content increased with elevation and slope length.


Fig. 3. Cross-correlation function of cotton lint yield, volumetric soil water, clay, sand, and elevation across a lag distance of ± 180 m on the 50% ET transect and 75% ET transect. Data were measured in 1998.

State-Space cotton lint yield models
The state-space analysis quantified how cotton lint yield measured in 1998 was related to soil water, clay, and elevation in space, and how strongly lint yield at position i was spatially based on previous measurement of lint yield, soil water, clay or sand, and elevation at position i-1. The multivariate state-space equations are shown in Fig. 4. The 95% confidence limits of estimates for yields at position i given by the state-space models are compared with measured yield values at the previous position i-1 along the two transects in 1998. Both multivariate state-space equations demonstrated that cotton lint yield yi, was positively weighted on soil water, and negatively weighted on elevation at previous position i-1 on both transects. However, lint yield yi was negatively weighted on the clay content Ci-1 along 50% ET transect, but positively weighted on the sand content Si-1 along 75% ET transect. Differently, 50% ET yield at position i is much more influenced by the previous site elevation compared to the 75% ET yield at position i.
As illustrated in Fig. 4, all lint yield measured in 1998 on both transects were within the 95% confidence limits (Fig. 4ab). The solid lines in the center of the shaded 95% confidence limit represent the prediction of the state-space equations. The autoregressively predicted values based on lint yield measured in 1998 varied with distance across the field (Fig. 4ab), depending on soil water, texture, and elevation. Higher lint yield was predicted on lower positions (center field area), and lower yield could be expected on the northern side of the field, where upslope and summit areas have less sand content.
The forecasted yield values of Yi obtained by the autoregressive state-space model with the lint yield data measured in 1998 were then related to cotton lint yield measured in 1999. The correlation between the lint yield measured in 1999 and predicted values of Yi is 0.72 and 0.74 for the 50% ET and 75% ET transect, respectively. The autoregressive state-space model appeared to be a successful forecast tool to predict future yields related to soil properties and field conditions.


Fig. 4. State-space cotton lint yield model at 50% ET irrigation (a) and 75% ET irrigation (b) with i, Location; Y, yield (kg ha-1); W, soil water (mm m-1); C, clay (g kg-1); S, sand (g kg-1); E, elevation (m); and e, model noises.

DISCUSSION

Environmental impact on soil water and lint yield variability
The most significant characteristics in variability of cotton response to irrigation and N fertilization were that soil water, NO3-N and particle size distribution, N uptake and lint yield were associated with site elevation and slope length. Irrigation amount appeared to be the most limiting factor causing differences in lint yield and N uptake. Water was not evenly redistributed, although irrigation was uniformly applied across the field. Water redistribution was primarily limited by particle-size, elevation, and slope length, which contributed to modify the spatial pattern of surface soil water evaporation and downward movement of soil water. As a result, the amount of soil water supply differed with soil depths and distance. Higher cotton lint yield and N uptake were therefore measured on lower positions, where soils contained more water received by runoff water from higher elevations. Inversely, lower cotton lint yield and N uptake were measured on northern upslope and summit area, where soils were drier because of possible wind erosion and water runoff. Weather variability also contributed to influence cotton response to irrigation and N fertilization within years. Effect of N fertilization was significant on lint yield in 1999. As compared to 1998, there was an increase of 14% of lint yield with 50% ET but a decrease of 4% with 75% ET irrigation in 1999. Our results showed that changes in soil water were more consistent than NO3-N, and were also widely controlled by microtopography.

State-space description of cotton lint yield variability
As a result of spatial association, the cross-correlation distance between cotton lint yield, soil water, clay or sand content, and site elevation was as short as 10 m, which indicated that the interdependence of soil and cotton crop variables and topography was within 10 m. Cotton lint yield variability, described by multivariate autoregressive state-space models, quantified that lint yield at i position was heavily weighted on soil water and site elevation at previous position i-1 On the 50% ET transect, measured lint yield (1998) decreased toward the upslope area, so that lint yield at position i was negatively weighted on yield at the previous position i-1, and the heavily negative weight of site elevation was therefore significant. On the 75% ET transect, lint yield at i position was positively weighted on the yield at the previous position i-1, since the measured lint yield tended to increase on lower and shoulder areas. As a result, the influence of site elevation at position i-1 on the lint yield at position i was more significant on the 50% ET than on the 75% ET, where sites are on average 0.4 m lower than sites on the 50% ET transect.
The positive weight on soil water and negative weight on elevation indicated that lint yield increases at position i were weighted on an increase of soil water and decrease of elevation. Clay on the 50% ET and sand on the 75% ET at the previous position i-1 had a significance weight on lint yield at position i. Clay content was high on the 50% ET northern upslope area, and sand content was important on the 75% ET center field, where subsurface soil contained more water through water infiltration and resulted in higher lint yield because of better root development and water and N use.
Future cotton yield in such a large field are weighted on soil water and topography variability as shown by the autoregressive state-space process. Higher lint yields would be expected on lower positions where wind-erosion hazard should be slight and accumulation of water and nutrient is expected. Low lint yields are predicted on upslope areas, where wind erosion hazard should be great and the soil is susceptible to loss of water and nutrients through wind removal. In the state-space cotton lint yield models, yields are primarily water-limited, and site elevation, slope length, and particle-size influence water use by affecting its distribution including transportation, infiltration, and surface water evaporation.

CONCLUSIONS

The role of soil water, texture, topography, and weather variability was attributed to their influence on cotton response to irrigation and N fertilization practices. Topography and soil texture were the key factors causing soil water redistribution variability and created differences in lint yield and N uptake in a large field. Irrigation had significant effects on soil water content, lint yield, and N uptake. The consistent pattern of soil and crop variables was measured with higher cotton lint yield and N uptake on lower positions. Lower soil productivity was linked to the upslope positions, where soils are more susceptible to water and wind erosion. The crosscorrelation distance between lint yield, soil water, clay, sand and elevation varied between 60 and 80 m. The state-space approach identified processes underlying crop yield variability in heterogeneous soils. Lint yield can be forecasted using a state-space model. State-space description of crop yield variability helped to understand the complex relationship between crop yield, soil properties, topography, and irrigation and fertilization practices. A variable irrigation and N fertilization adopting to soil property and topographical conditions could contribute to improve water and N use by crop in large fields.

Objective 3: To assess the use of remote sensing for site-specific management of cotton and water in a large field.

Water and N availability are commonly recognized as limiting factors for cotton production in the semiarid southern High Plains of Texas, where wind erosion is a hazard affecting soil water and nutrient use. There is a need for variable application of water and N related to field topographic features such as site elevation and slope length on the High and Rolling Plain soils. Characterization of the spatial plant/soil reflectance pattern could be used for a variable irrigation and N rate application across undulating fields. Soil type, plant cover, presence of moisture and organic matter, and landscape surface roughness can impact soil and plant reflectance in the visible red and near infrared wavelength regions. Plant reflectance, near infrared and red reflectance ratio, and NVDI varied with stress events, sun angles, cultivars, and irrigation and fertilization treatments. Landscape factors caused variation in plant and soil reflectance signal Cotton canopy ground cover and remotely sensed scene reflectance measured in Texas and California was linearly correlated. Plant and soil reflectance characteristics could be used to irrigation and fertilization management in cotton. Understanding the relationship between the spectral characteristics and the various cropping practices used in cotton production is key for further use of remote sensing as a tool for irrigation and fertilization management. For this study we hypothesized that irrigation and N fertilization rates, and topography and soil texture variability should influence water distribution and thus cotton spectral and agronomic response. The objectives of the study were (i) to measure cotton/soil reflectance related to different rates of irrigation and N fertilizer, (ii) to determine cotton/soil spectral and agronomic characteristics, and (iii) to assess influence of soil water distribution, topography, and soil texture in cotton reflectance, spectral vegetation index, plant water use, N uptake, and lint yield.

MATERIALS AND METHODS

Experimental setup and irrigation
The field study began in May 1998 at the Lamesa Agricultural Research Farm of Texas A&M University. The experimental area was 32 m wide and 700 m long. Altitude at the experimental site declines from S toward the center, then gently rolling upward to the N. The soil is structureless, well drained, highly erodible, and classified as an Amarillo sandy loam. The experimental treatments consisted of irrigation at rates of 50% and 75% calculated cotton evapotranspiration (ET), and N fertilization at rates of 0, 90 and 135 kg ha-1, respectively. There were 5 replications of N arranged in an incomplete block design. Plot size was 16 1-m rows wide and 50 m long. Cotton (cv. 'Roundup® Ready 2326') was seeded at a rate of 16.8 kg ha-1 on 8 May 1998 and on 10 May 1999. Fertilizer N (urea, 32-0-0) was fractionally chiseled into the soil at a rate of 45 kg ha-1 at emergence, bloom, and first square.
Weather conditions were either extremely dry in 1998 and periodically wet and dry in 1999. Water was applied with a center pivot using a LEPA. Total irrigation applied was 242 and 323 mm in 1998, and 190 and 286 mm in 1999 for the 50% ET and 75% ET, respectively.

Multi-spectral reflectance measurement
Multi-spectral plant/soil reflectance was measured using a portable 16-channel radiometer. Distance from sensors to crop surface was 2 m. With a 28° fov the sensor viewed an area 1.0 m in diameter. Spectral measurements were taken twice per week within a 15-30° solar zenith angle. Multispectral reflectance was measured over harvest rows from four areas, two were 25 m apart on row 10, and the other two were also 25 m apart on row 11 of each plot. A 0.5 x 0.5 m. Leaf area index (LAI) was simultaneously measured using a Plant Canopy Analyzer.

Soil water monitoring, and plant and soil sampling and analysis
To monitor soil water content, neutron access tubes were installed 25 m apart along a transect for each irrigation level. There were four neutron access tubes per plot. Monthly soil water content was measured by neutron attenuation. Nitrogen uptake by cotton was measured at a 10 d interval from early vegetative stage to harvest. Ten plants per plot were taken at the vegetative stage and four plants per plot were taken during bloom and boll stages. Plant density was measured at the vegetative and bloom growth stage at each plot. Cotton lint was hand harvested in each plot, and lint yield was determined from four 4-m2 areas at reflectance measurement locations. Soil samples were air-dried and sieved to 2 mm. Soil texture was measured using the hydrometer method. Root fresh plant biomass, and dry matter of roots, leaves, stems, bolls, and seeds were measured with samples dried at 70 °C for 48 h, and ground to a 40-mesh size. Plant N concentrations were determined using a LECO Analyzer.

Calculations, data statistics, and mapping
Volumetric soil water content was obtained by converting neutron probe readings using field calibration equations. The NDVI was determined from reflectance measured in the near infrared (NIR) and red (R) bands. Effects of irrigation and N fertilizer rates on cotton spectral and agronomic variables were tested using mixed model procedure. Regression and correlation relationships between these spectral and agronomic variables were determined using the General Linear Models. The NDVI map was generated using NDVI data measured in a 2 x 6 m grid across the plots using MapInfo 6.0 software.

RESULTS

Spatial pattern of soil water, and cotton plant reflectance
The spatial pattern of the soil water content varied with irrigation level, soil depth, and distance across the field (Fig. 1). Surface soil was drier than subsurface soil and soil water was distributed heterogeneously across the field as illustrated by the volumetric soil water content measured on 31 Aug. 1998 (Fig. 1ab). Along the 50% ET plots (Fig. 1a), lower water contents were measured between 350-450 m where the upslope area is located (Fig. 2a). Along the 75% ET plots (Fig. 1b), higher soil water contents were measured between 200-400 m, which was the lower elevation area (Fig. 2b).


Fig. 1. Spatial pattern of soil water content, soil and crop surface temperature and reflectance at center 661 nm, 810 nm, and 1650 nm at 50% and 75% ET irrigation level, respectively. Data were measured on 17 Aug. 1998.

Spatial distribution of the reflectance in the visible red, near infrared (NIR) and mid infrared (MIR) bands attributable to spectral soil and plant properties varied as a function of spatial soil water distribution, where the spectral measurements were taken at plant maturity on 17 Aug. 1998. Similar to the water distribution pattern, NIR reflectance was significantly higher on 75% ET plots (46 %) than 50% ET plots (42 %). Correspondingly, the red reflectance and MIR were significantly lower on the 75% ET plot, than the 50% ET plots. From south to north across the field, the consistent trend of soil water content and spectral characteristics was that the NIR reflectance was higher, but soil and crop surface temperature and visible red reflectance were lower where soil water content was higher (Fig. 1).

Spatial pattern of reflectance related to topography and soil texture
The multi-spectral curves show the soil and cotton reflectance as a function of topography (Fig. 2). The slope extended longer in the northern summit on the 50% ET area, and lower position extended in the middle on the 75% ET area (Fig. 2ab). The spectral reflectance, measured at plant maturity on 24 Aug. 1999 showed a close dependence on site elevation (Fig. 2).


Fig. 2. Spatial pattern of soil and crop surface temperature, and reflectance at center 661 nm, 810 nm, and 1650 nm related to site elevation in 50% and 75% ET irrigation area, respectively. Data were measured on 24 Aug. 1999.

As compared to the 50% ET (Fig. 2c), reflectance in the NIR band was higher, and soil and crop surface temperature and red and MIR reflectance were significantly lower on the 75% ET plots (Fig. 2d), showing also a similar trend as in 1998 (Fig. 1cd).

Temporal pattern of soil and cotton plant reflectance
The spectral curves show the soil, plant, and water reflectance as a function of wavelength (l) and crop development (Fig. 3). In June, red reflectance was higher (Fig. 3a), which corresponded to a higher percentage of the exposed soil at the early vegetative stage. As cotton grew, red radiance decreased and reflected NIR increased from June to August (Fig. 3a). The peak of NIR reflectance was measured in mid-Aug. at plant maturity. As a result of a decrease in LAI near the time of boll opening, reflected NIR decreased and the red increased in September (Fig. 3a).
The spectral curves also show the soil and plant reflectance as a function of irrigation level. Dry soil (Fig. 3b) reflected highly in the red and increased to the MIR band. The plants absorbed more blue and red energy than green, and strongly reflected NIR energy compared to the dry soil. Compared to 50% ET, there was a relatively higher absorption of blue, red, and MIR energy, and higher reflection of green and NIR energy on the 75% ET plots (Fig. 3b). Most of these differences in reflectance can be attributed to differences in canopy density. Higher NIR reflectance and lower red and MIR reflectance can be translated to more plant growth with lower water stress. The slight differences in the two red and two NIR can be attributed to the difference in the spectral width of these bands.


Fig. 3. Temporal patterns of seasonal soil and cotton crop multispectrum during the growing season (a), and comparison of dry soil and irrigated cotton reflectance (b). Data were measured in 1998.

Temporal pattern of cotton plant water use, N uptake and NDVI
The N uptake showed a S-shape growth pattern from vegetative stage to mid September and then collapsed, as demonstrated by the measurements taken in 1999 (Fig. 4ab). The N uptake collapse point indicated plant defoliation on day of year (DOY) 260 (Fig. 4ab). The NDVI was also followed a S-shaped pathway then collapsed (Fig. 4ab). In 1998 the maximum average NDVI was 0.69 and 0.76 at the 50% and 75% ET, respectively, determined on DOY 229. In 1999 the maximum average NDVI was 0.68 and 0.73 at the 50% and 75% ET, respectively, determined on DOY 236.


Fig. 4. Temporal patterns of the N uptake and NDVI measured in 1999.

The temporal pattern of LAI was similar to that of NDVI. The LAI varied between 0.12 and 2.68 during the two seasons. Both NDVI and LAI increased proportionally with increasing irrigation level (Fig. 5). The NDVI curve showed a normal distribution pattern whose peak was situated towards the end August. High NDVI value was a result of an increase of reflectance in the NIR band.


Fig. 5. Temporal patterns of the NDVI and LAI measured in 1998.

Mixed effects of irrigation on reflectance characteristics
Mean cotton lint yields were 704 and 962 kg ha-1 in 1998, and 819 and 924 kg ha-1 in 1999 at 50% and 75% ET irrigation level, respectively. Weather variability might have caused lint yield difference within years since the 1998 season was exceptionally dry but unusually wet in June 1999. The random effects of the mixed model variance components (block, and block x treatments) were not significant.

Correlation of spectral and agronomic parameters and field heterogeneity

Cotton spectral characteristics were linked to site elevation and soil texture. Soil and crop surface temperature, and visible and MIR reflectance were positively correlated to site elevation and clay content, but negatively to sand content. As a result, NIR reflectance, NDVI, and NIR and red ratio (NIR/R) were negatively correlated to site elevation. The negative or positive correlation coefficients indicated that high lint yield; N uptake and plant water content were associated with low elevation and high sand content. Red and MIR reflectance was significantly correlated to plant water content NIR reflectance, NDVI, and the NIR/ RED ratio were positively linked to lint yield, N uptake, and soil water.
Cotton lint yield was strongly related to NDVI. Lint yield increased linearly with an increase of NDVI at 50% ET and at 75% ET. In addition, spatial distribution of the NDVI status was heterogeneous across plots (Fig. 7). As a result of a significant interaction between irrigation and N fertilizer on near IR reflectance, NDVI was obviously higher on 90 kg N ha-1 plot (Fig. 6a) than on 135 kg N ha-1 plot (Fig. 6b). The 75% ET plots had higher NDVI values with higher frequency as compared to the 50% ET plot. The heterogeneous distribution of the NDVI reflected the impact of topography and soil texture on soil water distribution and plant growth.


Fig. 6. Near infrared and red reflectance-based NDVI map. Spectral reflectance data were measured on 15 Aug. 1998.

DISCUSSION

Soil water, texture, and topography variability and cotton spectral characteristics
The most significant characteristics in spectral reflectance variability were their association with soil water distribution, site elevation, slope length, and soil texture. Water was not evenly distributed in the soil. The amount of soil water appeared to be the most limiting factor in spectral cotton characterization. Increasing water in the soil reduced reflectance in the visible and MIR bands but increased reflectance in the near IR band, which was related to an increase of plant water content, fresh biomass, and N uptake. Therefore a decrease of visible radiance and increase of near IR reflectance would mean higher soil and plant water content. The association of spectral reflectance with the topography was marked by an increase of reflectance in the visible region and a decrease in the NIR reflectance shoulder at 810 nm on higher positions, where plant density was significantly reduced. The plant density was higher on lower positions than on northern upslope areas, and plant fresh biomass had the same trend with higher values on lower areas and lower values on higher areas. In June 1999 there was evidence of water erosion with rye grass residues and younger plants pulled off from upslope to footslope area due to the rain. The decline of plant density led to an increase of soil exposure. Therefore soil and crop surface temperature and red reflectance became important, but near IR reflectance decreased on upslope areas, where there was less plant coverage. Inversely, near IR reflectance increased on lower positions in center field areas, where plants were dense with more soil water.

Spectral and agronomic responses to irrigation and N fertilization
Irrigation practice and interaction between irrigation and N fertilization appeared to be the most limiting factors affecting cotton reflectance. As compared to the 50% ET, irrigation at the 75% ET level supplied to the soil an adequate amount of water since its near IR, NDVI, LAI, lint yield, and N uptake were significantly higher. Since 1994, N fertilizer at a rate of 190 kg ha-1 per year has been applied to this field. In early spring, soil residual NO3-N in the rooting depth were 134 and 121 kg ha-1 in the 50% ET and 75% ET plots, respectively. Therefore there were no significant spectral and agronomic responses to N fertilizer due to the large amount of residual soil NO3-N. Also, variability in rain might have caused the differences in interaction between irrigation and N fertilization on cotton spectral response between years. In 1998 total seasonal
The most significant relations between cotton spectral and agronomic characteristics were that NIR and reflectance varied primarily as a function of plant fresh biomass, MIR reflectance changed with plant water content, and the NDVI and NIR/RED ratio were also good indices to predict N uptake, lint yield, and plant water content. Also, the heterogeneous distribution of NDVI was a result of heterogeneous distribution of soil water, site elevation and soil texture within a short distance across the undulating field. Our results showed that a variable water and N application adopted to local field conditions would be realizable by using such a NDVI map calibrated for local conditions.

CONCLUSIONS

Irrigation practice, interaction between irrigation and N fertilization, and variability in soil water, texture, microtopography and weather significantly affected the spectral reflectance properties of a cotton crop. The NIR reflectance increased and red reflectance decreased significantly with increasing irrigation, plant fresh biomass, and N uptake. The MIR reflectance decreased with increasing plant water content. Soil and crop surface temperature was cooler on the 75% ET plots, where higher plant reflectance and lower soil reflectance were measured. Presence of soil moisture and sand reduced reflectance in the visible and MIR band, and increased reflectance in the NIR band. Soil and cotton surface temperature, visible, NIR and MIR reflectance, and soil water distribution, plant water, N uptake by cotton, and lint yield were linked to site elevation and slope length. Red reflectance was higher on higher site positions where slope was also extended. Slope length increased reflectance in the visible, and MIR band. Spectral reflectance can be used with NDVI mapping to identify spatial patterns in soil water and N across heterogeneous field. Characterization of spatial distribution of spectral plant and soil properties can be the basis for developing technologies for variable irrigation and N rate application across heterogeneous field.

Objective 4 Integrate the effect of N, H20, and insect injury on growth, development, maturation, and yield of cotton.
.
Lamesa:
The most common arthropod species found using visual leaf samples were pests including cotton aphid, whitefly, and thrips along with green lacewing larvae and spiders (both predators). The most common arthropods from sweep net samples were pests including boll weevil adults and armyworm larvae along with predators such as spiders, ladybugs, green lacewing adults, big-eyed bugs, and nabids. Finally, boll weevil and bollworm caused most of the injury to squares and bolls. We examined the data using 3 different methods: (1) ANOVA to determine the effects of irrigation and nitrogen fertilizer levels on yield, % plant nitrogen, and insect distributions and damage, (2) multiple regression to determine possible correlations between insect and plant parameters and cotton yield, and (3) examination of insect spatial distributions to determine the size of management units for variable pesticide application.
Results of ANOVA indicate the lint yield was significantly greater at the high irrigation level than at the low irrigation level, but that fertilizer level had no effect on yield (Fig. 1). (Significance in all ANOVAs was set at P < 0.05.) The opposite was true for % nitrogen in the cotton leaves, % nitrogen was higher at the low irrigation level than at the high irrigation level, and % nitrogen increased significantly with increasing nitrogen fertilizer levels (Fig. 2). Results for ANOVAs on aphid density and bollworm square damage were similar as that of % plant nitrogen; both were significantly lower at the high irrigation level than at the low level. Also, in all cases the irrigation level by nitrogen fertilizer level interaction was significant indicating that nitrogen fertilizer had no effect at the low irrigation level but did have an affect at the high irrigation level, with the medium and high fertilizer levels having higher aphid and bollworm damage than the low fertilizer level (Figs. 2, 3, and 4). For bollweevil boll damage the irrigation level by nitrogen fertilizer interaction was also significant, because at the low irrigation level bollworm damage was significantly less at the low fertilizer level (than the medium or high levels), but at the high irrigation level bollworm damage was less at the high fertilizer level than at either the medium or low level (Fig. 5).


Figure 1. The effects of irrigation and nitrogen fertilizer levels on lint yield (g) .


Figure 2. The effects of irrigation and nitrogen fertilizer levels on % plant nitrogen.


Figure 3. The effects of irrigation and fertilizer levels on aphid density (3/leaf).


Figure 4. The effects of irrigation and nitrogen fertilizer levels on the proportion of squares damaged by bollworm.

We also used multiple correlation analyses to determine which variables-plant (height, % nitrogen); soil (pH, electrical conductivity) or landscape (latitude, longitude, altitude, block, nitrogen fertilizer level, and irrigation level) had the greatest impact on aphid density, bollworm square damage, and bollweevil boll damage. Results indicated that aphid density was significantly correlated with soil pH (r = -0.228), elevation (r = -0.125), and irrigation level (r = -0.278) and that the overall R2 value was very low (0.13) indicating that these factors explained only about 13% of the variation in aphid density in cotton. Bollworm square damage was significantly correlated with pH (r = -.116), electrical conductivity (r = 0.229), latitude (r = 0.191), plant height (r= 0.316), block (r = 0.24), and irrigation level (r = -0.194) with an overall R2 value of 0.221 indicating that the factors examined explained about 22% of the variation in bollworm damage. Finally, bollweevil boll damage was significantly correlated with pH (r = -.325), electrical conductivity (r = 0.235), latitude (r = 0.116), longitude (r = -0.242) plant height (r = 0.176), nitrogen fertilizer level (r = -0.129), block (r = 0.132), and irrigation level (r = -0.666) with an overall R2 value of 0.73 indicating that the factors examined explained about 73% of the variation in bollweevill damage.


Figure 5. The effects of irrigation and nitrogen fertilizer levels on the proportion bolls damaged by boll weevils.

Finally, we examined the distribution patterns of aphids, bollworm damaged squares, and bollweevil damaged bolls using different sized sampling units to determine whether insect clumping would allow for the use of insecticides in only portions of the field. For aphids we found that if 150 m by 24 row areas (blocks) were examined to determine if the aphid density was above the spray threshold (> 50 / leaf), insecticides would only need to be applied to 50% of the field. If smaller areas, 50 m by 24 rows, are examined than only 38% would be sprayed, while if even smaller areas (25 m by 12 rows) are examined the difference becomes small (37%) indicating that 50 m by 24 row areas are the best size for aphid management units. This is somewhat different for bollworm damage, for 50 m by 24 row areas only 50% of the field would need be sprayed; for the 25 m by 24 row areas 45% would be sprayed, and for the smallest area 30% would need to be sprayed. Bollweevil damage was similar to bollworm, using the large areas, 100% would need to be sprayed, if the medium sized areas were used as management units then 88% would need to be sprayed, while if the small areas were used then only 58% would need to be sprayed. For bollworm and bollweevil, the smallest area would be the best size for the management unit.
In conclusion, our findings indicate that variable irrigation and nitrogen fertilizer used in precision agriculture will affect insect pest densities and damage to cotton fruits. Also, because nitrogen fertilizer and irrigation levels affect pest densities, both can be used in a model for predicting pest densities on a large scale. However, on a smaller scale, pest densities were only slightly correlated with plant, soil, and landscape factors indicating that these factors alone will not be useful in predicting pest densities. Finally, the clumped distribution pattern of insects and insect damage indicates that it should be possible to apply pesticides to only portions of a field, thus allowing the survival of natural enemies and the slowing of insecticide resistance evolution in unsprayed areas.
Lubbock:
ANOVA results for lint, seed, and carpel weights were similar; therefore, we'll just discuss lint and seed weights (per 2 m-row treatment plots) rather than repeating the same information for all three. Both irrigation level and damage type (real or simulated) had significant effects on yield. Yield was greater at the high irrigation level (means = 56.8 mg lint /plot and 90.9 mg seed /plot) than at the low irrigation level (means = 37.8 mg lint /plot and 58.2 mg seed /plot). Yield was also greater for real damage (means = 48.8 mg lint /plot and 76.9 mg seed /plot) than for simulated damage (means = 45.8 mg lint /plot and 72.3 mg seed /plot). The latter was probably due to high bollworm mortality in real damage plots. This caused yield to be greater in real damage plots than in simulated damage plots, because we didn't take bollworm mortality into account when simulating bollworm damage levels at the three densities. This was done to ensure that we obtained some information on damage even if high mortality in the real damage plots caused treatment densities to be indistinguishable.
There were significant interactions between irrigation level and density and between irrigation level and infestation period (Fig. 6 and 7). Neither density nor infestation period significantly affected yield at the high irrigation level (Fig. 6). However, at the low irrigation level, yield decreased with increasing bollworm density (Fig. 7). Also, yield was significantly less for the last infestation period (boll maturation) than at either of the earlier infestation periods (Figs. 6 and 7).
We have 4 main conclusions from the Lubbock bollworm fruit consumption-injury study. First and most simply increasing the level of irrigation increases yield. Second, simulated bollworm damage effects yield significantly more than real bollworm damage when bollworm mortality is not included in simulations. Third and most importantly, increasing bollworm density decreases yield when irrigation is low but has no effect when irrigation is high. Finally, bollworm damage that occurs during the boll maturation period decreases yield (in comparison with earlier cotton developmental periods) when irrigation is low, but not when irrigation is high.


Figure 6. The effects nitrogen fertilizer level, damage level, and damage timing, on lint yield in cotton at the high irrigation level.


Figure 7. The effects nitrogen fertilizer level, damage level, and damage timing, on lint yield in cotton at the low irrigation level.

Leaf nitrogen content was estimated using a chlorophyll (SPAD) meter to measure the concentration of chlorophyll in the leaves. Results from comparison of Dr. Chilcutt's chlorophyll (SPAD) meter readings and Dr.'s Li and Lascano nitrogen analysis indicate that SPAD measurements were not a good indicator of cotton plant nitrogen content. Also, mean SPAD readings were similar for the 3 nitrogen fertilizer levels, with no significant differences between levels. However, SPAD readings did vary significantly with time. After an initial decrease in SPAD readings from June to July, SPAD readings increased steadily from July to September. Finally, SPAD readings were not correlated with yield or with insect densities or insect damage to cotton squares or bolls, indicating that the SPAD meter is not a useful instrument for measuring crop variability or insect variability in cotton.

Objective 5 Develop decision support software that predicts the site-specific impact of multiple stresses on cotton crop growth, development, maturation, and yield

College Station and Beaumont:
A major aspect of our research/extension program has focus on the development of decision support software for cotton. The resulting software, Scout Master for Cotton, has been developed to quickly and efficiently summarizes field scouting data for electronic delivery to producers, private consultants, agribusiness, university scientists, and other agencies and organizations that use IPM information. Scout Master currently allows Extension Agents to provide information via newsletters on pest, beneficial, and crop status, and pesticide, water, and fertilizer use across a large number of fields or across a series of sample dates for any field in Scout Master's database. It also allows for computer access to large quantities of organized field data that can form a readily accessible historical database. This allows pest population development to be compared between and among years, for different regions, and for different pest management methods. As Scout Master's use continues to expand, the data will become useful for evaluating resistance management programs, evaluating regional and area-wide management approaches, and providing valuable information on the region-level movement of potentially damaging pest populations.
Scout Master is also a valuable tool for training new Extension Agents on how to systematically sample fields in terms of monitoring pest species and crop growth and development, how to store data, and how best to deliver data to growers and crop consultants. The speed and degree with which sound pest management practices are adopted by crop consultants and growers in a crop production regions is greatly determined by the quality of the regions Extension Agents.
During 1997, Scout Master for Cotton was tested on about 300 fields by extension agents-IPM. Based on suggestions made by extension agents and their scouting teams, numerous changes and additions were made and version 2.0 of Scout Master for Cotton was released in spring 1998. Additions included expansion of the graphics options and inclusion of pesticide, water, and fertilizer record keeping. During 1998, Scout Master for Cotton was tested on nearly 3,000 fields, in the Rio Grande Valley, the Coastal Bend, the Southern Rolling Plains, and the High Plains of Texas. Follow-up meetings produced requests for additional improvements, which are being incorporated and release of version 3.0 occurred in June 1999. These latest additions included options for improved field sorting to facilitate ease of data entry, options for sorting data to enable comparison of insect abundance as affected by planting date, irrigation management, and plant variety, an expanded export utility, and a utility to import geo-referenced data from global positioning systems (GPS). Our current efforts with Scout Master for Cotton's include several additions, including 1) a weed management component, 2) integrating pest thresholds, and 3) incorporating PDA (Palm pilot) technology to greatly expanding Scout Master's ease of use, and speed with which it can be used. We have investigated the use of voice-recognition software but have found the error rate for existing software to be too expensive and multiple licenses excessively expensive.
Additions and improvements have rapidly expanded Scout Master for Cotton's data management options and its ease of use. With each new season, the program will be tested across an increasingly greater area of Texas. The Scout Master development team will continue to meet a minimum of twice per year, once prior to crop planting and once following harvest, to summarize needed corrections and desired additions. Corrections and additions will continue to be incorporated into Scout Master for Cotton as requested.
In addition to our regular Scout Master meetings, the Scout Master for Cotton developmental team has met with cotton experts to develop crop stage specific control thresholds for each of the major pest species. The incorporation of PDA-based data entry and Scout Master database synchronization has the potential to greatly increase the speed with which field scouting data can be entered into Scout Master.
The net result of this component of our project will be improved decision making by growers resulting in increased net profits and decreased risks to human health and the environment. Results of the project will also facilitate agribusinesses in providing improved service to agricultural customers and serve as an evaluation tool for regional and area wide management efforts. Further developments will result in the creation of computerized databases that will serve as valuable resources that can be used to optimize crop production and management inputs.

B. Education/technology transfer

· Held a workshop titled "State-Space Analysis and Other Statistics and its Application to Precision Agriculture" at the Lubbock center, 30 Nov - 2 December, 1998. Dr. D. R. Nielsen and Dr. Ole Wendroth instructed the workshop. R. J. Lascano organized the workshop.

· Held a meeting with Dr. Basil Acock from the USD-ARS crop simulation unit to implement the use of their cotton simulation model CPM (Cotton Production Model). This model is geared towards the management of water and nitrogen and fits within our objectives. We are currently evaluating the model and expect that it will be used as a management tool.

· The CPM model is not adequate for the environmental conditions of the Texas High Plains and we are now using the Cotton2K model from A. Marani. We are currently evaluating this model. This model will provide a management tool that can be used for site-specific management.

C. Milestones Achieved:

· During the 1998 and 1999 growing seasons, lint yield did not respond to nitrogen fertilizer due to the high content of residual nitrate-nitrogen in the soil. This indicates that the current recommendation of only sampling the surface 6 inches for soil nutrient management is erroneous and can lead to over fertilization and unnecessary cost.

· Results from both growing seasons indicate that it is possible to estimate plant leaf area and plant biomass from plant reflectance measurements. The reflectance measurements are made with a hand-held radiometer.

· Our state-space analysis indicates that it is possible to use this statistical approach to identify management units within a field. Results indicated that we can explain lint yield variability with a 95% confidence using state-space analysis. This analysis shows that in Lamesa lint yield variability is related to irrigation amount, elevation and soil N-NO3 content. These results are significant because they indicated that lint yield for a given location can be maximized using the state-space equations. For example, irrigation water in combination with N fertilizer can be variably applied on the field to maximize lint yield. Also noteworthy, is that in Lamesa NO3 content in the soil is not related to yield due to its very high concentration.

· Variability in site elevation, slope length, and soil texture may affect crop response to irrigation and fertilization. A two-year (1998-1999) study was conducted in a center pivot irrigated cotton field on the semiarid Southern Texas High Plaines to determine cotton lint yield and N uptake pattern related to irrigation, N fertilization, and field conditions. Treatments consisted of irrigation at 50% and 75% calculated cotton evapotranspiration (ET), and N input at rates of 0, 90, and 135 kg ha-1 arranged in an incomplete block design. Soil, water and crop variables were measured as a function of irrigation, fertilization, and space along transects. Higher soil water content, cotton lint yield, and N uptake were linked to lower positions in the field. Mixed model analysis showed that main effects of irrigation were significant on increase of soil water content, plant water content, N uptake, and cotton lint yield (P > 0.0425-0.0012). The N input had no effect in a dry year (1998). The model residual was significant on all measured variables (P > 0.0001). In 1998, lint yield, soil water, clay, sand, and elevation were crosscorrelated at a lag distance of ± 30-40 m. The multivariate autoregressive state-space analysis quantified the underlying processes of cotton lint yield, soil water, clay, sand, and topography. Lint yield measured in 1999 was correlated to the forecasted data by the state-space model in 1998. Results showed that state-space description of crop yield variability helps to understand interdependency between irrigation, fertilization, and field heterogeneity.

· Variability in cotton multispectral reflectance could be attributed to irrigation and N fertilization practices and the impact of microtopography on water and N redistribution. A two-year (1998-1999) study was conducted in a center pivot irrigated field on the semiarid South Texas High Plains to determine cotton spectral reflectance characteristics related to irrigation, N fertilization, and field conditions. Treatments consisted of irrigation at rates of 50% and 75% calculated cotton evapotranspiration (ET), and N fertilization at rates of 0, 90 and 135 kg ha-1 arranged in an incomplete block design. The composite reflectance properties were investigated over a wavelength range of 447-1752 nm. Near infrared reflectance increased and visible red reflectance decreased with increasing irrigation. Reflectance in the red and mid infrared bands increased with site elevation. All cotton spectral characteristics were significantly affected by irrigation (P > 0.0020-0.0488), and interaction between irrigation and N input (P > 0.0001-0.0163). Spectral reflectance, normalized difference vegetation index (NDVI), plant water and N use, and lint yield were strongly associated with site elevation and soil texture. It is concluded that irrigation rates and landscape attributes caused variation in reflectance signal.

D. Publications:

· Lascano, R. J., R. L. Baumhardt, S. K. Hicks and J. A. Landivar. 1998. Spatial and temporal distribution of surface water content in a large agricultural field. 4th International Conference on Precision Agriculture, 19-22 July, 1998. St. Paul, MN, USA, Part A: 19-30.

· Li, Hong, R. J. Lascano, E. M. Barnes, and P. Waller. 1999. Multispectral remote sensing related to water and nitrogen use in cotton. 1999 Annual Meetings American Society of Agronomy, Salt Lake City, UT, 31 October - 4 November, 1999, p 21.

· Li. Hong and R. J. Lascano. 1999. State-space approach for management of field heterogeneity in cotton. Annual Meetings American Society of Agronomy, Salt Lake City, UT, 31 October - 4 November, 1999, p 202.

· Li, Hong, R. J. Lascano, Jill Booker, L. Ted Wilson, and K. F. Bronson. 2000. Cotton lint yield variability in a heterogeneous soil at a landscape level. Soil & Tillage Research 1553:1-14 (In press).

· Li, Hong, R. J. Lascano, Jill Booker, K. F. Bronson, E. Segarra, E. M. Barnes, and L. T. Wilson. 2000 Spectral reflectance characteristics of cotton related to soil water and topography variability. 5th International Conference on Precision Agriculture, 16-19 July, 2000 St. Paul, MN, USA (In press).

· Li, Hong, R. J. Lascano, Jill Booker, K. F. Bronson, L. T. Wilson, and E. Segarra. 2000. Underlying field heterogeneity on water and n use in cotton: State-space analysis. 5th International Conference on Precision Agriculture, 16-19 July, 2000 St. Paul, MN, USA (In press).

· Barnes, E.M., T. R. Clarke, S. E. Richards, P. D. Colazzi, J. Haberland, M. Kostrzewski, P. Waller, C. Choi, E. Riley, T. Thompson, R. J. Lascano, Hong Li, and M. S. Moran. 2000. Coincident detection of crop water stress, nitrogen status and canopy density using ground-bases multispectral data. 5th International Conference on Precision Agriculture, 16-19 July, 2000 St. Paul, MN, USA (In press).

· Li, Hong, R. J. Lascano, E. M. Barnes, Jill Booker, L. T. Wilson, K. F. Bronson and E. Segarra. 2000. Multispectral reflectance characteristic of cotton related to soil water, texture, and topography. (Paper submitted to the Agronomy Journal).

· Lascano, R. J. and H. Li. 2001. Precision farming to improve water use (Invited paper to be published in the Encyclopedia of Water Science, in review).

· Li, Hong, R.J. Lascano, J. Booker, L. T. Wilson, and K. F. Bronson. 2000. Landscape-scale assessment of soil texture, water, plant reflectance, and lint yield variability in irrigated cotton. Annual Meetings American Society of Agronomy, Minneapolis, MN, 5-9, November, 1999, p 324.
· Wilson, L. T., R. Huffman, T. Fuchs, J. Wang. 1997.Master cotton. Versions 1.0, 1.2, 1.3 for Windows 95/NT systems. Copyright, Texas A&M University.
· Huffman, R., T. Fuchs, L. T. Wilson, J. Wang, M. Wallace, B. Baugh, R. Minzenmayer, and John Norman. 1998. "Scoutmaster": New cotton insect data entry and analysis software for Windows 95. 1998 Proceedings Beltwide Cotton Production Research Conferences. pp. 168-170.
· Wilson, L. T., R. Huffman, Mike Wallace, and T. Fuchs. 1998 Scoutmaster for cotton. Versions 2.0 for Windows 95/NT systems. Copyright, Texas A&M University.
· Knutson, A., and L. T. Wilson. The beat bucket: A rapid reliable method for sampling predatory insects and spiders in cotton. 1998 Proceedings Beltwide Cotton Production Research Conferences. pp.

E. Precision agriculture proposals:

· Remote Sensing and in-situ Measurement of Soil Water in Precision Agriculture: Integration of Measurements with Simulation Models. Robert J. Lascano. Amount requested $339,000 for three years. Proposal submitted to the Idaho National Environmental and Engineering Laboratory (INEEL). (Proposal was funded for three years). 1997-1999.
· Remote Sensing Detection of Multiple Biotic and Abiotic Stresses in Precision Agriculture. R. J. Lascano, PI with the collaboration of C. M. Rush, G. J. Michels, T. L. Archer, K. F. Bronson and L. T. Wilson. Proposal submitted to NASA for $598,271 for three years. (Proposal was not funded), 1998.

· Water Management of Cotton in Precision Agriculture: Lint Yield as a Function of Site-Specific Irrigation. Robert J. Lascano. Amount requested $60,000 for one year. Proposal submitted to the Cotton State Support Committee. (Proposal was funded for three years), 1999-2001

· Data set for Cotton Production Model. Robert J. Lascano. Amount requested $4,330 for one year. Proposal submitted to the USDA-ARS. (Proposal was funded), 1999.

· A state-space approach to manage low energy precision application (LEPA) irrigation systems. Robert J. Lascano, and D. Porter. Amount requested $182,500 for two years. Proposal submitted to the Advanced Research Program, Texas Higher Education Coordination Board. (Proposal was not funded). 1999.

· Yield Tracker: A yield mapping and prediction information delivery system. S. J. Maas, R. J. Lascano and D. Cooke. Proposal submitted to the IFAFS- USDA-CSREES. (Proposal was funded at $800,000 for three years).
· Huffman, Wilson, 1996-1997. Development of a geo-referenced component for Scout Master for Cotton, and development of Scout Master for Corn. TPMA. $40,000. (proposal was funded)
· Wilson, Fuchs, 1998-1999. Development of a database component for Scout Master for Cotton. TDA. $25,000 (Proposal was funded)
· Wilson, Fuchs, 1999-2000. Incorporating Voice-Recognition, Insect Thresholds, and Weed Monitoring into Scout Master for Cotton. TDA $31,000 (Proposal was funded).
· Wilson, Norman, et al. 1998-1999. Incorporating voice recognition, insect thresholds, and weed monitoring into Scout Master for Cotton. TPMA. $41,000. (Proposal was not funded)
· Wilson, Fuchs, 1998-2000. Scout Master for Cotton. TDA. $20,000 (Proposal was not funded).
· Wilson, Fuchs, et al. 1999-2000. A multidisciplinary approach to integrating multiple species thresholds into a holistic cotton management system. Southern Region IPM. (Proposal was not funded).

F. Precision Agriculture meetings attended/papers (posters) presented:

· Lascano, R. J., R. L. Baumhardt, S. K. Hicks and J. A. Landivar. 1998. Spatial and temporal distribution of surface water content in a large agricultural field. Paper presented at the 4th International Conference on Precision Agriculture, 19-22 July, 1998. St. Paul, MN, USA, Part A: 19-30.
· Machado, S. E. D. Bynum Jr., D. T. Rosenow, G. C. Peterson, T. L. Archer, R. J. Lascano, K. Bronson, and E., Segarra. 1999. Spatial variability of sorghum yield: site-specific interactions of soil water and pests. 1999 Poster presented at the Sorghum Conferences, Tucson, AZ
· Li, Hong, R. J. Lascano, E. M. Barnes, and P. Waller. 1999. Multispectral remote sensing related to water and nitrogen use in cotton. Poster presented at the 1999 Annual Meetings American Society of Agronomy, Salt Lake City, UT, 31 October - 4 November, 1999, p 21.
· Li. Hong and R. J. Lascano. 1999. State-space approach for management of field heterogeneity in cotton. Poster presented at the 1999 annual Meetings American Society of Agronomy, Salt Lake City, UT, 31 October - 4 November, 1999, p 202.
· Lascano, R.J., J. Booker, H. Li, K. Bronson, W. Keeling, T. Wheeler, J. Bordovsky, T. Wilson, S. Searcy, E. Segarra, J. Gannaway, R. Boman, P. Dotray, N. Hopper, and H. Kaufman. 2000. Cotton precision agriculture work in the Texas High Plains. Paper presented at the 2000 Cotton Beltwide meetings in San Antonio, TX 4-8 January, 2000.
· Lascano, R.J., J. Booker, H. Li, K. Bronson, W. Keeling, T. Wheeler, J. Bordovsky, T. Wilson, S. Searcy, E. Segarra, J. Gannaway, R. Boman, P. Dotray, N. Hopper, and H. Kaufman. 2000. Precision agriculture in the Texas High Plains. Paper presented at the 2000 Cotton Beltwide meetings in San Antonio, TX 4-8 January, 2000.
· Li, Hong, R. J. Lascano, Jill Booker, K. F. Bronson, E. Segarra, E. M. Barnes, and L. T. Wilson. 2000 Spectral reflectance characteristics of cotton related to soil water and topography variability. Paper presented at the 5th International Conference on Precision Agriculture, 16-19 July, 2000 St. Paul, MN, USA (In press).
· Li, Hong, R. J. Lascano, Jill Booker, K. F. Bronson, L. T. Wilson, and E. Segarra. 2000. Underlying field heterogeneity on water and n use in cotton: State-space analysis. Paper presented at the 5th International Conference on Precision Agriculture, 16-19 July, 2000 St. Paul, MN, USA (In press).
· Barnes, E.M., T. R. Clarke, S. E. Richards, P. D. Colazzi, J. Haberland, M. Kostrzewski, P. Waller, C. Choi, E. Riley, T. Thompson, R. J. Lascano, Hong Li, and M. S. Moran. 2000. Coincident detection of crop water stress, nitrogen status and canopy density using ground-bases multispectral data. Paper presented at the 5th International Conference on Precision Agriculture, 16-19 July, 2000 St. Paul, MN, USA (In press).
· Li, Hong, R.J. Lascano, J. Booker, L. T. Wilson, and K. F. Bronson. 2000. Landscape-scale assessment of soil texture, water, plant reflectance, and lint yield variability in irrigated cotton. Poster presented at the annual Meetings American Society of Agronomy, Minneapolis, MN, 5-9, November, 2000, p 324
· Li, Hong, R. J. Lascano, E. M. Barnes, and P. Waller. 1999. Multispectral remote sensing related to water and nitrogen use in cotton. 1999 Poster presented at the Annual Meetings American Society of Agronomy, Salt Lake City, UT, 31 October - 4 November, 1999, p 21.
· Li. Hong and R. J. Lascano. 1999. State-space approach for management of field heterogeneity in cotton. Poster presented at the Annual Meetings American Society of Agronomy, Salt Lake City, UT, 31 October - 4 November, 1999, p 202.
· Lascano R. J. H. Li, and Jill Booker. 2000. Assessing soil and cotton lint yield variability on a landscape-scale. Keynote paper presented at the First Joint Congress of the Soil Science Society of America and the German Soil Science at Osnabrück, Germany 18-22 September, 2000.
· Lascano, R. J. Water Management of Cotton in Precision Agriculture: Lint Yield as a Function of Site-Specific Irrigation. Paper presented at the Cotton State Support committee, Lubbock 7-8 December, 2000.
· Wilson, Member, USDA/NRI Agricultural Systems Panel, 1997-1998.
· Wilson, Panel Manager, USDA/NRI Agricultural Systems Panel, 1998-1999.
· Wilson, Scout Master for Corn demonstration, Texas IPM Technical Advisory Committee meeting, October 1998.

G. Other Developments: